Acylcarnitine: Structure, Metabolism, Functions, and Advanced Analysis Techniques MetwareBio Carnitine Shuttle an overview ScienceDirect Topics Critical Role of Mitochondrial Fatty Acid Metabolism in Normal Cell Function and Pathological Conditions Short Note On Carnitine Shuttle: Fatty acids are oxidized in mitochondria, and synthesized in the cytoplasm. Long chain fatty acids must form an active intermediate, fatty acyl CoA, before being oxidized inside mitochondria. Catherine Acyl Carnitine Translocase: MCAT Guide to Fatty Acid Transport & Oxidation King of the Curve