Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Oral delivery of glutathione: antioxidant function, barriers and strategies ScienceOpen Glutathione synthesis in the mouse liver supports lipid abundance through NRF2 repression Nature Communications Liposomal Glutathione (Lemon Mint) BodyHealth BodyHealth.com LLC Is Tylenol Safe During Pregnancy? Risk Factors, Not Causation